《植物生理学报》 2014, 50(11): 1630-1638

森林草莓全基因组MADS-box转录因子基因的鉴定及凤梨草莓果实FaMADS1基因克隆

周厚成^1,2,*, 李刚¹, 赵霞¹, 康兆茹¹, 郭蔼光²

¹中国农业科学院郑州果树研究所, 郑州450009; ²西北农林科技大学生命科学学院, 陕西杨凌712100

通信作者：周厚成；E-mail: zhouhoucheng@caas.cn；Tel: 0371-65330972

摘　要：

本文利用生物信息学方法对森林草莓(Fragaria vesca)基因组数据库中MADS-box基因的数量、结构类型、序列特征及染色体定位进行分析。结果表明, 获得70个含SRF-TF结构域的森林草莓FvMADS基因, DNA长度289~14 596 bp, 编码66~1 437个氨基酸残基, 有21个FvMADS没有内含子, 在7条染色体上呈不均匀分布; 68个FvMADS蛋白序列含有保守基序Motif 1, 最佳匹配序列为“RQVTFSKRRNGLLKKAYELSVLCDAEVALIIFSSRGKLYEF”。另外, 从栽培品种‘丰香’草莓果实中克隆了FaMADS1基因, 该基因属于MADS-box基因家族, cDNA全长1 167 bp, 编码区750 bp, 推导编码249个氨基酸, 具有MADS结构域和K-box结构域。

关键词：草莓; MADS-box; 转录因子; 生物信息学; FaMADS1基因

收稿：2014-06-30   修定：2014-09-22

资助：河南省基础与前沿技术项目(132300410034)和公益性行业(农业)科研专项(201003064-7)。

Genome-Wide Sequence Identification of MADS-Box Transcription Factor Gene Family from Fragaria vesca and Cloning of FaMADS1 gene from F. ananassa Fruit

ZHOU Hou-Cheng^1,2,*, LI Gang¹, ZHAO Xia¹, KANG Zhao-Ru¹, GUO Ai-Guang²

¹Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China; ²College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China

Corresponding author: ZHOU Hou-Cheng; E-mail: zhouhoucheng@caas.cn; Tel: 0371-65330972

Abstract:

In this paper, MADS-box genes were obtained by Fragaria vesca genome database query and named FvMADS. MADS-box genes family, including the number of the FvMADS genes, structure type, sequence characteristic and chromosomal localization, was analyzed by using bioinformatics methods. The results showed that 70 FvMADS genes including SRF-TF structure domain were obtained. These FvMADS gene family DNA sequence were 289–14 596 bp in length and encoded 66–1 437 amino acid residues, and 21 FvMADS genes had no introns. These genes were not evenly distributed on 7 chromosomes. 68 FvMADS proteins contained a conserved base sequence Motif 1 of the best matching sequences for “RQVTFSKRRNGLLKKAYELSVLCDAEVALIIFSSRGKLYEF”. In addition, the FaMADS1 gene from F. ananassa cv. ‘Toyonoka’ fruit was cloned. The gene which belonged to MADS-box gene family, the full-length cDNA of 1 167 bp encoding 249 amino acids, was derived by MADS and K-box structure domains.

Key words: Fragaria; MADS-box; transcription factor; bioinformatics; FaMADS1 gene